Cytotoxicity Assay Kit- What You Need to Know



A cytotoxicity assay kit is one that can be relied upon for colorimetric assay tests. It is extremely useful as far as measuring lactate dehydrogenase (LDH) quantitatively is concerned. It assesses the extent of release of lactate dehydrogenase into the media with the source in this case being damaged cells. The results of this text act as biomarkers for processes such as cytolysis and cytotoxicity in cells.

Specifications of cytotoxicity assay kit
A cytotoxicity assay kit is normally compatible with mammalian cells. It should ideally be used with microplate readers. It has a colorimetric detection method, as has been said already. It can be used with both 96-well plates as well as 384-well plates. One such kit can be used to study in the region of 200 reactions.
It can be used in an add-mix-read assay format, especially in the context of suspension and adherent cells. It measures lactate dehydrogenase release in a quantitatively by forming colored products. It is a robust instrument, one that uses stable lactate dehydrogenase enzyme activity in the role of a cytotoxic marker. It is pretty flexible as well and can jolly well be used for screenings with high throughput rates. One of its major advantages is that it is not radioactive.
It is generally regarded as a safer substitute for cytotoxicity assays that release 51 Cr-release.
How does it work?
A cytotoxicity assay kit is used to measure the extracellular lactate dehydrogenase that has been released into culture media. For this purpose the kit uses an enzymatic reaction that leads to the formation of a red formazan product. This particular product can be measured in a spectrophotometric manner.
What is lactate dehydrogenase?
Lactate dehydrogenase or LDH is basically cytosolic enzyme, which indicates the level of toxicity at a cellular level.
What does a kit include?
A cytotoxicity assay kit is inclusive of substrate mix, stop solution, assay buffer, lactate dehydrogenase positive control, and 10X lysis buffer.
What does it need to work?
In order to work these kits require microplate readers that can read absorbance at the rate of 680nm and 490nm. It should ideally be a 96-well plate with a clear flat bottom. It is also ideal if the microplate is capable of working with multichannel pipettes and spectrophotometers.
Areas of application
A cytotoxicity assay kit can be used for measurement of in vitro cytotoxicity that has been mediated by chemicals, siRNA, immune cells, and microRNA. It can also detect cytotoxicity in 3D tissue engineering applications and bioreactors.      
  
        

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