Cytotoxicity Assay Kit- What You Need to Know



A cytotoxicity assay kit is one that can be relied upon for colorimetric assay tests. It is extremely useful as far as measuring lactate dehydrogenase (LDH) quantitatively is concerned. It assesses the extent of release of lactate dehydrogenase into the media with the source in this case being damaged cells. The results of this text act as biomarkers for processes such as cytolysis and cytotoxicity in cells.

Specifications of cytotoxicity assay kit
A cytotoxicity assay kit is normally compatible with mammalian cells. It should ideally be used with microplate readers. It has a colorimetric detection method, as has been said already. It can be used with both 96-well plates as well as 384-well plates. One such kit can be used to study in the region of 200 reactions.
It can be used in an add-mix-read assay format, especially in the context of suspension and adherent cells. It measures lactate dehydrogenase release in a quantitatively by forming colored products. It is a robust instrument, one that uses stable lactate dehydrogenase enzyme activity in the role of a cytotoxic marker. It is pretty flexible as well and can jolly well be used for screenings with high throughput rates. One of its major advantages is that it is not radioactive.
It is generally regarded as a safer substitute for cytotoxicity assays that release 51 Cr-release.
How does it work?
A cytotoxicity assay kit is used to measure the extracellular lactate dehydrogenase that has been released into culture media. For this purpose the kit uses an enzymatic reaction that leads to the formation of a red formazan product. This particular product can be measured in a spectrophotometric manner.
What is lactate dehydrogenase?
Lactate dehydrogenase or LDH is basically cytosolic enzyme, which indicates the level of toxicity at a cellular level.
What does a kit include?
A cytotoxicity assay kit is inclusive of substrate mix, stop solution, assay buffer, lactate dehydrogenase positive control, and 10X lysis buffer.
What does it need to work?
In order to work these kits require microplate readers that can read absorbance at the rate of 680nm and 490nm. It should ideally be a 96-well plate with a clear flat bottom. It is also ideal if the microplate is capable of working with multichannel pipettes and spectrophotometers.
Areas of application
A cytotoxicity assay kit can be used for measurement of in vitro cytotoxicity that has been mediated by chemicals, siRNA, immune cells, and microRNA. It can also detect cytotoxicity in 3D tissue engineering applications and bioreactors.      
  
        

Location: 3191 Corporate Pl, Hayward, CA 94545, USA

Comments

Post a Comment

Popular posts from this blog

All about cholesterol assay kit and more

Image
A cholesterol assay kit is referred to as a colorimetric assay which is used to calculate the total amount of cholesterol present in tissue, plasma, homogenates and serum. Today, we will be taking a look at the basics of Cholesterol Assay Kit . Make sure you’re reading on to find out more in this regard. The assay is based on the reaction governed by enzyme and this particular reaction is capable of measuring free cholesterol as well as cholesterol esters. Now, assay kits as we all understand is a very important part of scientific researches. It is so important to ensure that you’re procuring your kit from reliable assay kit providers who have a long history of offering quality assay solutions to the science industry. What should you keep in view as a researcher? Researchers also require zeroing in on providers who are capable of catering to the needs of the highly demanding science industry. It is important to actually remember that you cannot really go on to ch
Read more

β-N-Acetylglucosaminidase Assay Kit

Image
β-N-Acetylglucosaminidase  (NAG) is a lysosomal enzyme involved in a variety of biological processes such as the degradation of glycoproteins and glycolipids, cell proliferation, and signal transduction. NAG is found in many tissues in the body, but due to its high molecular weight, it can not be filtered through the glomerular membrane. For this reason, in the presence of tubular damage or a glomerular lesion, urinary NAG activity increases. Elevated NAG levels in urine are an early indication of renal damage, such as injury due to diabetes mellitus, inflammation, nephritic syndrome, urinary tract infection, and more. Various forms of cancer have been associated with increased levels of NAG in serum. Genetically inherited lipid storage disorders, such as Tay-Sachs and Sandhoff disease, arise from deficiencies of the enzyme. BioAssay Systems non-radioactive, colorimetric NAG assay is based on the cleavage of  p -nitrophenol from a synthetic substrate.  p -Nitrophenol becomes int
Read more

Alanine Transaminase Assay Kit

Image
BioAssay Systems scientists have over 30 years of extensive experiences in assays and services. Our services encompass a wide variety of custom projects. BioAssay Systems provides highest possible quality and the most convenient bioassays which is non-radioactive formats, and safe for health. We commite for customers safety and satisfaction, so we develops products in non-radioactive formats such as absorbance, fluorescence and luminescence detection techniques. We  provides,  markets innovative and high-throughput assay solutions. Bioassay kits are simple and convenient to use, which is high quality and convinent to use. Our products are superior in performance and require little to no time for assay optimization. Alanine Transaminase Assay Kit Alanine Transaminase  (ALT), known as serum alanine aminotransferase (ALAT) or pyruvic transaminase (SGPT), facilitates the conversion of alanine and a-ketoglutarate to pyruvate and glutamate. ALT plays an important role in glucon
Read more