About Aconitase Assay Kit
An Aconitase assay kit is a direct and simple assay kit, which happens to be highly sensitive as well as compatible with HTS. It can also be described as a colorimetric assay that can be used to measure Aconitase activity that takes place in a biological sample. How does the assay work? In the Aconitase assay ki t the citrate is changed to isocitrate by using aconitase. Then, this is processed further and it creates a product that can change a probe, which is almost colorless, into a form that is highly colored and has a λmax at 450nm. This product is proportional to the amount of enzymatic activity that happens in a human body. This kit uses a wide array of samples to determine aconitase activity. Once again, a coupled enzyme reaction is used to carry out the entire process. A single unit of aconitase is good enough to isomerize 1.0 μmole of citrate each minute with a pH of 7.4. However, the whole process needs to be conducted in a temperature of exactly 25 degrees Ce...