About Aconitase Assay Kit



An Aconitase assay kit is a direct and simple assay kit, which happens to be highly sensitive as well as compatible with HTS. It can also be described as a colorimetric assay that can be used to measure Aconitase activity that takes place in a biological sample.

How does the assay work?

In the Aconitase assay kit the citrate is changed to isocitrate by using aconitase. Then, this is processed further and it creates a product that can change a probe, which is almost colorless, into a form that is highly colored and has a λmax at 450nm. This product is proportional to the amount of enzymatic activity that happens in a human body. This kit uses a wide array of samples to determine aconitase activity. Once again, a coupled enzyme reaction is used to carry out the entire process. A single unit of aconitase is good enough to isomerize 1.0 μmole of citrate each minute with a pH of 7.4. However, the whole process needs to be conducted in a temperature of exactly 25 degrees Celsius.   

Details of Aconitase assay kit

One piece of Aconitase assay kit can be used to conduct 100 tests. It basically determines enzyme activity. It is used for samples such as cell culture supermatants, plasma, urine, various biological fluids, serum, and tissue extracts. It takes only 40 minutes for this assay kit to complete one function. It is used in functional studies in the form of a dilution dependent on assays.

What is aconitase?

The scientific name of aconitase is aconitate hydratase. It is described as an iron-sulfur protein that contains a [Fe4S4]2+ cluster. This cluster plays a catalytic role in stereospecifically isomerizing citrate and thus changing it to isocitrate in the process. This is done by way of cis-aconitate in what is known as the tricarboxylic acid cycle. This is basically a non-redox-active procedure. There is a couple of aconitases in the tissue – cytosolic (c-) and mitochondrial (m-). These enzymes are related but still quite different from each other. They have also been identified in eukaryotes. They are also coded to be used in the context of different chromosomes.

When cells and other kinds of biological samples show loss of aconitase activity upon being treated by using pre-oxidants it is regarded as a kind of oxidative damage. It is said that aconitases can be inactivated owing to oxidative stress. However, the process can be reversed. 
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