Bioassay system

The 5-AMP-activated protein kinase (AMPK) is a key sensor of intracellular energy balance. AMPK is activated in response to an increase in the AMP/ATP ratio which can be caused by a number of factors such as muscle contraction, starvation, or hypoxia. AMPK is a heterotrimeric protein complex comprising of α - (63 kDa), β - (38 kDa) and γ - (38 kDa) subunits. For each subunit, isoforms have been identified ( α 1, α 2, β - 1, β - 2, γ 1, γ 2, γ 3) which theoretically allow the formation of 12 different proteins. The α -subunit contains a serine/threonine kinase domain and the regulatory subunits contain binding sites for AMP and ATP and for glycogen. AMPK is activated by phosphorylation on Thr-172 within the catalytic domain. AMP binding results in a 2 to 5-fold increase in AMPK activity compared to the basal level. Binding of AMP to the α -subunit causes allosteric activation of the kinase and induces a conformational change in the kinase domain that protects AMPK from dephosphoryla...

BioAssay Systems scientists have over 30 years of extensive experiences in assays and services. Our services encompass a wide variety of custom projects. BioAssay Systems provides highest possible quality and the most convenient bioassays which is non-radioactive formats, and safe for health. We commite for customers safety and satisfaction, so we develops products in non-radioactive formats such as absorbance, fluorescence and luminescence detection techniques. We  provides,  markets innovative and high-throughput assay solutions. Bioassay kits are simple and convenient to use, which is high quality and convinent to use. Our products are superior in performance and require little to no time for assay optimization. Alanine Transaminase Assay Kit Alanine Transaminase  (ALT), known as serum alanine aminotransferase (ALAT) or pyruvic transaminase (SGPT), facilitates the conversion of alanine and a-ketoglutarate to pyruvate and glutamate. ALT plays an important ...