About Caspase Assay Kit
The Caspase assay kit uses colorimetric method for detection. One such kit can conduct 100 tests. It normally functions in the context of cell lysate samples and determines enzyme activity. It takes almost two hours to complete one test. Description A caspase assay kit deals with the functioning of caspases, which work in accordance with the sequence DEVD. It also provides a way of assaying that is easy and simple at the same time. This assay works on a principle known in the scientific world as spectrophotometric detection and deals with chromophore p-nitroaniline, also referred to as p-NA. This is done after it separates from DEVD-pNA, which also happens to be a labeled substrate. How does the detection happen? One can quantify the p-NA light emission by using either a microtiter plate reader or a spectrophotometer. They can be used at either 405 or 400 nm. When one compares how much p-NA has been absorbed from an apopt...