Bioassay system

AMMONIA  (NH 3 ) or its ion form ammonium (NH 4 + ) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH 3  are very desirable. BioAssay Systems ammonia/ammonium assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity ( λ ex/em = 360/450nm) is proportional to the ammonia concentration in the sample. Application • For quantitative determination of ammonia/ammonium concentration by fluorimetry at 360nm/450nm (compatible on handheld fluorimeter catalog# FL360450). Key Features • Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia. • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method •...

Acetate   is a common anion and fundamental to all forms of life. When bound to coenzyme A, it is central to the metabolism of carbohydrates and fats. It is acid form, acetic acid, is produced and excreted by acetic acid bacteria, such as Acetobacter genus and Clostridium acetobutylicum, which are found universally in foodstuffs, water, and soil. Acetic acid is also a component of the vaginal lubrication of humans and other primates, where it appears to serve as a mild antibacterial agent. Acetic acid is the main component of vinegar, and extensively used in food, dyes, paints, glue and synthetic fibres. BioAssay Systems assay uses enzyme-coupled reactions to form a colored, fluorescent product. The color absorbance at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the acetate concentration in the sample.