Ammonia Assay Kit


AMMONIA (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH3 are very desirable. BioAssay Systems ammonia/ammonium assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (λex/em = 360/450nm) is proportional to the ammonia concentration in the sample.



Application
• For quantitative determination of ammonia/ammonium concentration by fluorimetry at 360nm/450nm (compatible on handheld fluorimeter catalog# FL360450).

Key Features
• Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia.

• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method
• FL360/450nm

Samples
• Biological (eg urine) and environ-mental samples

Species
• All

Procedure
• 15 min

Size
• 200 tests

Detection Limit
• 12 μM

Shelf Life
• 6 months
Location: 3191 Corporate Pl, Hayward, CA 94545, USA

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