Alcohol Dehydrogenase Assay Kit


ALCOHOL DEHYDROGENASE (ADH) is an oxidoreductase which catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the breakdown of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation. BioAssay Systems non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.


Application
• For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism.

Key Features
• Fast and sensitive. Linear detection range (20 μL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
• OD565nm
Samples
• Biological (e.g. plasma, serum, urine, tissue and culture media)
Species
• All
Procedure
• 30 min
Size
• 100 tests
Detection Limit
• 0.4 U/L
Shelf Life
• 6 months

BioAssay Systems scientists have over 30 years of extensive experiences in assays and services. Our services encompass a wide variety of custom projects. BioAssay Systems provides highest possible quality and the most convenient bioassays which is non-radioactive formats, and safe for health. Please Visit : https://www.bioassaysys.com/
Contact Us: 510-782-9988


Location: 3191 Corporate Pl, Hayward, CA 94545, USA

Comments

Post a Comment

Popular posts from this blog

Amylase Assay Kit – all the critical points of interest

Image
The amylase Assay Kit is utilized as a bit of interest to perform colorimetric territory. It can be utilized as a bit of an expansive combination of tests that might be recorded as underneath: •              Cell culture supernatant •              Urine •              Serum •              Plasma •              Other common liquids •              Tissue Extracts •              Cell culture media It takes 40 minutes to lead one test with this specific unit. To the degree species reactivity is concerned it responds with mice, individuals, and rats. It is relied upon to work with warm blooded animal things as well. This colorimetric measure pack is utilized to see an amylase action by procedure for a response that uses a couple of means. An amylase divider ought to disengage the substrate known as ethylidene-pNP-G7. This is done recollecting a definitive goal to make parts that are more diminutive in nature. It is a-glucosidase that as time goes on changes
Read more

AMPK Phosphorylation Assay Kit

Image
The 5-AMP-activated protein kinase (AMPK) is a key sensor of intracellular energy balance. AMPK is activated in response to an increase in the AMP/ATP ratio which can be caused by a number of factors such as muscle contraction, starvation, or hypoxia. AMPK is a heterotrimeric protein complex comprising of α - (63 kDa), β - (38 kDa) and γ - (38 kDa) subunits. For each subunit, isoforms have been identified ( α 1, α 2, β - 1, β - 2, γ 1, γ 2, γ 3) which theoretically allow the formation of 12 different proteins. The α -subunit contains a serine/threonine kinase domain and the regulatory subunits contain binding sites for AMP and ATP and for glycogen. AMPK is activated by phosphorylation on Thr-172 within the catalytic domain. AMP binding results in a 2 to 5-fold increase in AMPK activity compared to the basal level. Binding of AMP to the α -subunit causes allosteric activation of the kinase and induces a conformational change in the kinase domain that protects AMPK from dephosphoryla
Read more

Assay Solutions: A basic idea

Image
The science industry is always in need of highly effective assay solutions. Assaying is a process – rather a chemical analysis- where metals are tested for the presence or strength of components that are already present in them. Needless to say, assaying is highly important for scientific researches. A few basic ideas about assay kits Based on the researchers’ needs (or rather the demands of the scientific research itself), researchers can choose from among several assay kits. A few are mentioned below: Ø   ATPAssay Kit   Ø   CaspaseAssay Kit Ø   CholesterolAssay Kit Ø   CytotoxicityAssay Kit Ø   FattyAcid Assay Kit  Ø   Glucoseassay Kit  Ø   GlutathionePeroxidase Kit Ø   KinaseAssay Kit What you need to do However, researchers are required taking an important stand when it comes to selecting a company offering assay solutions. Now, with the increasing demand for assay kits in scientific research, the number of companies offering these solution
Read more