Aldehyde Dehydrogenase Inhibitor Screening Kit



Bioassay System

BioAssay Systems scientists have over 30 years of extensive experiences in assays and services. Our services encompass a wide variety of custom projects. BioAssay Systems provides highest possible quality and the most convenient bioassays which is non-radioactive formats, and safe for health.

ALDEHYDE DEHYDROGENASES (ALDHs) are a superfamily of oxidoreductases which catalyze the conversion of aldehydes to carboxylic acids. ALDH is crucial in the metabolism of alcohol as alcohol dehydrogenase breaks down ethanol to acetaldehyde. Acetaldehyde, which is toxic to the body, is in turn broken down by ALDH to acetic acid. Imbalances of aldehyde dehydrogenase have been linked to both alcoholism and alcohol sensitivity in people. Inhibitors of the enzyme have been used in cases to treat alcoholism in patients. Cancer stem cell populations also display a heightened activity of ALDH, making ALDH inhibition a promising anti-cancer therapy approach. BioAssay Systems QuantiChrom™ Aldehyde Dehydrogenase Inhibitor Screening Kit is based on the enzymatic conversion of acetaldehyde to acetic acid and NADH by ALDH. The formed NADH in turn reduces a formazan reagent into a colored product the absorbance of which, measured at 565 nm, is proportional to the enzyme activity in the reaction. The percent inhibition of a test compound can be determined by comparing the activity of ALDH treated with a test compound to the activity of untreated ALDH.


Application
•  HTS for inhibitor screening and evaluation of aldehyde dehydrogenase inhibitors.

Key Features
•  Rapid and reliable. Can be completed in under an hour.

•  Homogenous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.

•  Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method
•  OD565nm

Samples
•  Aldehyde dehydrogenase

Species
•  All

Procedure
•  30 min

Size
•  100 tests

Shelf Life
•  6 months
Location: 3191 Corporate Pl, Hayward, CA 94545, USA

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