Acetylcholine Assay Kit
ACETYLCHOLINE is
a neurotransmitter produced in acetylcholinergic neurons. It plays important
roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as
well as in learning, memory and mood. BioAssay Systems method provides a
simple, direct and high-throughput assay for measuring acetylcholine in
biological samples. In this assay, acetylcholine is hydrolyzed by
acetylcholinesterase to choline which is oxidized by choline oxidase to betaine
and H2O2. The resulting H2O2 reacts
with a specific dye to form a pink colored product. The color intensity at
570nm or fluorescence intensity (530/585 nm) is directly proportional to the
acetylcholine concentration in the sample.
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