About Adipolysis Assay Kit



Adipolysisassay kits are used in order to screen compounds that are involved in lipid storage as well as are related to metabolism. These contain reagents that are required in order to induct adipogenesis in an in vitro manner. This releases glycerol that has been accumulated in stored lipid droplets. This kit also measures the glycerol thus released in an accurate manner. This product can be described as a total assay that can be used to study how lipolysis happens in triglycerides. It is also able to demarcate cells that are like adipocyte. It is also capable of dealing with quantitation of glycerol that has been released.

Differentiating 3T3-L1 cells

The Adipolysis assay kit is capable of differentiating 3T3 –L1 cells as well. For this purpose it uses a procedure that is used quite commonly in adipogenesis induction. As an end part of this process it collects liquid droplets. It also uses isoproterenol in the form of a positive control. In this process it releases glycerol from triglycerides. The kit uses a process of incubation in order to measure the amount of free glycerol that is there in the cell culture medium. For this purpose elements such as glycerol kinase, horseradish peroxidase, and phosphate oxidase are used when colorimetric substrates are present.         

The end result

All this helps in generating a chromophore that can be detected at 540 nm. The extent of glycerol that is thus released is related to both the extent of lipolysis and triglyceride stored. This makes the kit very useful for investigators that screen compounds thus having an effect on metabolism or lipid storage.

Benefits of the kit

The Adipolysis assay kit has sufficient reagents for assaying a single plate of supernatants containing 96 wells and releasing glycerol in the process. One can also alternatively assay how liquid droplets have gathered by using either the Adipogenesis Assay Kit or the Lipid Droplets Fluorescence Assay Kit. One may also employ the Glycerol Cell-Based Assay Kit in order to study the release of glycerol.

Why is it important?

This is an important tool in the context of obesity, which may be regarded as the single-biggest health problem facing human beings nowadays, considering the fact that it contributes to so many diseases like atherosclerosis and diabetes, which can never really be cured across the lifespan of the affected but only controlled to a certain extent. This kit helps identify pathways for greater fatty tissue formation in one’s body and also helps in reversing lipid stores in the said tissues. This increases chances of stopping obesity in its tracks.        
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